antibodies against phosphospecific well nonphospho-ampkα acc  (Cell Signaling Technology Inc)

Journal: Nutrition & Metabolism

Article Title: AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model

doi: 10.1186/1743-7075-3-31

Figure Lengend Snippet: Effect of AICAR on phospho-status of ACC, AMPK during adipocyte differentiation . The differentiating 3T3-L1 cells were treated with different concentrations of AICAR (from 0.1 to 1.0 mM) and proteins were isolated at various time periods (from 0 to 9 days). Immunoblot analysis for p-ACC, p-AMPKα, AMPKα1, AMPKα2 and β actin was examined as described in Materials and Methods (a). 3T3L1 cells were differentiated as above in the presence or absence of AICAR (0.5 and 1.0 mM)/iodotubercidin (0.1 μM) for 9 days. Cells were processed for the immunoblot analysis for p-ACC and β actin (b).

Article Snippet: Antibodies against phosphospecific as well as nonphospho-AMPKα and ACC were from Cell Signaling (Beverly, MA).

Techniques: Isolation, Western Blot

Journal: Nutrition & Metabolism

Article Title: AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model

doi: 10.1186/1743-7075-3-31

Figure Lengend Snippet: Effect of AICAR on phospho-status of ACC, AMPK and AMPK enzymatic activity in AICAR treated STD and DIO treated mice . Total protein from epididymal fat content from 6 weeks AICAR treated STD and DIO treated mice was isolated. Immunoblot analysis for p-ACC, p-AMPKα, AMPKα1 and β actin was examined as described in Materials and Methods (a). The enzymatic activity of AMPKα1 was examined by immunoprecipitating using anti-AMPKα1 followed by assessing its activity using SAMS peptide as described in Material and methods (b). *** p < 0.001; ** p < 0.01 as compared to STD mice group. $ p < 0.05, as compared to DIO vehicle treated group.

Article Snippet: Antibodies against phosphospecific as well as nonphospho-AMPKα and ACC were from Cell Signaling (Beverly, MA).

Techniques: Activity Assay, Isolation, Western Blot